Determination of oligonucleotides by XtimateC18 HPLC Column
Test Report
Chromatography conditions:
Column |
Welch Xtimate® C18（4.6×250mm，5um） |
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Mobile Phase |
A: Phosphate buffer/methanol=95/5; B: Phosphate buffer/methanol=75/25 |
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Column Temperature |
25 ℃ |
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Detector |
UV |
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Injection Volume |
10μL |
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Flow Rate |
1.0 ml/min |
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Detect wavelength |
260nm |
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Elution procedure |
|
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Note |
/ |
Chromatogram and Data:
Retention time |
K’ |
Area(nAU*S) |
Height(mAU) |
Symmetry Factor |
Peak width(min) |
Number of theoretical plates |
Resolution |
Selectivity |
5.925 |
- |
19.6584 |
2.97282 |
1.04 |
0.1033 |
18216 |
- |
- |
7.441 |
- |
65.75619 |
6.43044 |
1.05 |
0.1600 |
11985 |
6.76 |
1.26 |
11.945 |
- |
1086.99316 |
73.28335 |
1.03 |
0.2275 |
15279 |
13.66 |
1.61 |
18.117 |
- |
2350.23511 |
116.62537 |
1.12 |
0.3109 |
18803 |
13.47 |
1.52 |
Conclusion:
Under these HPLC conditions. Welch Xtimate® C18（4.6×250mm，5um）can meet the customer's requirements.