Desalt Sample? G25 Purification Column Helps
Tandex G25
Tandex G25 is a kind of bead medium formed by 3 chlorine-1,2 propylene oxide cross-linked dextran, which is suitable for desalination and buffer replacement of biological macromolecules with relative molecular weight of more than 5000. Tandex G25 can provide coarse, medium, fine and ultrafine particles. If the particle is smaller, the resolution will be higher and the flow rate will be slower simultaneously. Average volume of samples on different media is about 30% of the column volume. Yield of protein at multiple particle size on Tandex G25 decreases with the increase of column length, and the yield of short column with large inner diameter is higher when the column volume keeps the same.
Technical parameters
Application
Specification
| Pre-column |
BC-10 Desalting gravity column |
PreCot G25M Pre-column |
PreLoad 16/10 Pre-column |
| Column bed volume | 8.3ml | 5ml | 20ml |
| Medium | Tandex G25M | Tandex G25M | Tandex G25M |
| Dimension | 1.54*4.46cm | 1.6*2.5cm | 1.6*10cm |
| Max.volume of sample | 2ml | 1.2ml | 5ml |
| Exclusion range (globulin, Mr) | 5000 | 5000 | 5000 |
| Flow rate（cm/h） | Gravity flow | <150 | 150 |
| pH stability | 2-13（lone term） | 2-13（lone term） | 2-13（lone term） |
Note: PreCot series and PreLoad series pre-columns use 1/16 inch interface, which allows it used with injectors, pumps, AKTA and other medium and low pressure chromatography system.
