Hard to separate polycyclic aromatic hydrocarbons? Use Wisys 5000

September 19, 2019

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Why to separate polycyclic aromatic hydrocarbons?

Polycyclic aromatic hydrocarbons are volatile hydrocarbons produced when coal, petroleum, wood, tobacco, organic polymer compounds and other organic substances are not completely burned. They are environmental and food contaminants, which are widely found in the atmosphere, water and soil. And more than 200 polycyclic aromatic hydrocarbons that have been discovered are carcinogenic, so we need to detect polycyclic aromatic hydrocarbons in the environment and food.

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The result on common C18 column by a HPLC instrument.

Except the peak of naphthalene, anthracene, decene, other peaks can not meet the requirements. And even there’s no peak for a kind of substance.

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Method

Use Wisys 5000 HPLC system and Ultisil PAH 4.6×250mm, 5μm.

Ultisil PAH is a special column for detecting polycyclic aromatic hydrocarbons, which is developed according to EPA610.

Wisys 5000 is a high performance liquid chromatograph developed by Welch, which has intelligent and systematic innovation, high flexibility and applicability.

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Chromatography condition

Sample information

Name: 16 polycyclic aromatic hydrocarbons；

Description: Mixed standard solution: A2S，00826-MXC009M100ANAE，100 μg/ml；

Reference standard: HPLC method.

Column: Welch Ultisil PAH, 4.6×250mm, 5μm

Detector: 220nm

Temperature: 30℃

Injection：10μl

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Chromatogram and database