LP-C18 : Can it Separate Isomers and Chiral Diastereomers?
Following the previous article “1Can LP Columns Only Resist Strong Acids?” this article takes a user-centric approach, guiding everyone in a clear and in-depth exploration to understand the manufacturer’s design principles for chromatography columns. Behind it lies many “treasures” — the mysterious and unknown applications.
In our experiments, we unexpectedly discovered that LP-C18 exhibits astonishingly unique performance in the separation of isomers and chiral diastereomers. It truly challenges and refreshes our understanding!
Case 1: Separation of Isomers of Amino Acids
Chromatography Column: Ultisil® LP-C18 (4.6×250mm, 3μm).
Mobile Phase: Potassium dihydrogen phosphate – Acetonitrile – Methanol;
Flow Rate: 1.0 mL/min;
Injection Volume: 10μL;
Column Temperature: 65℃;
UV Detector: 214nm.
Explanation: In the amino acid sequence of the drug intermediate, one amino acid is Asp, and the impurity is β-Asp. After trying chromatography columns such as XS-C18, XB-C18, and C30, there was no separation trend observed. Only LP-C18 demonstrated the ability to separate them.
Case 2: Separation of Chiral Compounds
Chromatography Column: Ultisil® LP-C18 (4.6×250mm, 5μm).
Mobile Phase: Sodium dihydrogen phosphate – Sulfobutyl-β-cyclodextrin sodium salt – Methanol;
Flow Rate: 0.8 mL/min;
Injection Volume: 10μL;
Column Temperature: 30℃;
UV Detector: 205nm.
The above graph illustrates the separation of two chiral compounds. Compared to chiral columns, LP-C18 offers a more cost-effective solution!
Case 3: Separation of Agomelatine Isomers, following USP 43
Chromatography Column: Ultisil® LP-C18 (4.6×250mm, 3μm).
Mobile Phase: Methanol/Water;
Flow Rate: 0.6 mL/min;
Injection Volume: 10μL;
Column Temperature: 50℃;
UV Detector: 259nm.
LP-C18’s fundamental packing structure and the simultaneous interaction of multiple forces enhance the potential for compound and isomer separation. In our experiments, when compared to commonly used isomer separation columns such as XS-C18, PFP, C30, and chiral columns, LP-C18 proved to be more economical, stable, and exhibited better reproducibility. It’s undeniable that chromatography is indeed a practical discipline!