Purify Antibodies? Welch Comes to Help

Monoclonal antibodies are widely used in the diagnosis, prevention and treatment of diseases. Since the first monoclonal antibody drug was launched in 1986, nearly 40 kinds of McAb drugs have been listed, and more than 300 kinds of McAb drugs have been in clinical study. McAb drugs have become one of the fastest-growing drug fields in the world.

In the production process of monoclonal antibody, the cost of McAb drugs can account for 50-70% of its production cost for that this process requires rigorously for the efficiency and cost. Protein A affinity medium is the most critical material for antibody separation and purification as its properties and cost determine the purity, yield and cost of McAb products.

Currently, majority of antibodies IgG molecules use Protein A affinity chromatography for purification. Protein A is the surface protein of cell wall found in staphylococcus aureus with a molecular weight of 42 kDa, and its five different binding regions can bind IgG Fc fragments with a strong specific affinity. Each Protein A molecule can bind at least two antibody IgG molecules.

---

Protein A Tanrose 4FF

Protein A Tanrose 4FF is the universal affinity chromatography medium for the separation and purification of monoclonal antibodies, polyclonal antibodies, or Fc- fusion labels. Protein A has five IgG binding regions and other regions with unknown functions. Recombinant protein A removes binding sites with albumin on cell surface and contains only five IgG binding regions, reducing nonspecific adsorption.

Based on highly cross-linked 4% agarose gel, Protein A Tanrose 4FF can be purified at a relatively high flow rate by monoclonal antibody and polyclonal antibody, which is generally accepted by industrial customers.

Name	Protein A Tanrose 4FF	Protein A Solid
Bead structure	6% highly cross-linked agarose	Highly rigid agarose
Particle size	45-165µm	45-120µm
Mean particle size	90μm	75µm
Binding capacity	＞20mg(humanIgG)/ml(media)	＞28mg(humanIgG)/ml(media)
pH stability*	3-9((long term)2-11(short term)	3-10((long term)2-12(short term)
Chemical stability*	8 M urea, 20% ethanol, 6 M guanidine hydrochloride (8 days), stable at pH 2-9.
Max. flow rate	250cm/h	500cm/h
Operating pressure	≤0.3MPa	≤0.5MPa
Storage solution	20% ethanol	20% ethanol
Storage temp.	4-8℃	4-8℃

Buffer Preparation

The water and buffer are recommended to be filtered with 0.22 μm or 0.45 μm filter membrane before use.

Equilibrium liquid/eluate : 20 mM Na₂HPO₄, 0.15M NaCl, pH 7.0;

Eluent: 0.1 M glycine, pH 3.0;

Neutralizer: 1 M Tris-HCl, pH 8.5.

Sample Purification

After packing the Protein A Tanrose 4FF, various conventional medium and low pressure chromatographic systems can be used.

Protein A Tanrose 4FF

P/N	Specificatoin
AF4020	5mL
AF4021	25mL
AF4022	200mL
AF4023	1L