Determination of arabinose and arabitol content using Xtimate Sugar-Ca
|
Column |
Welch Xtimate® Sugar-Ca（7.8×300mm，5μm) |
|
Mobile Phase |
Water |
|
Detector |
RID |
|
Injection Volume |
20 μL |
|
Flow Rate |
0.6 ml /min |
|
Column Temperature |
60℃ |
|
Note |
/ |
Chromatogram and Data:
- Mixed reference solution detection chart
|
Retention time(min) |
K’ |
Area(nRIU*s) |
Height(nRIU) |
Symmetry Factor |
Width (min) |
Number of plates |
Resolution |
Selectivity |
|
12.810 |
- |
1.38967e6 |
6.89644e4 |
0.91 |
0.3141 |
9214 |
- |
-- |
|
17.018 |
- |
1.30419e6 |
5.35491e4 |
0.91 |
0.3818 |
11013 |
7.11 |
1.33 |
Conclusion:
Under these chromatographic conditions, Welch Xtimate® Sugar-Ca（7.8×300mm，5μm) can meet the detection requirements.