Determination of mannitol content with Ultisil HILIC-Amide
Chromatography conditions:
|
Column |
Ultisil®HILIC Amphion II（4.6×250mm，5um） |
|
Mobile Phase |
Acetonitrile/water=75/25 |
|
Injection Volume |
10 μL |
|
Flow Rate |
1.0 ml /min |
|
Detector |
ELSD |
|
Column Temperature |
30℃ |
|
Detection wavelength |
ELSD detector temperature: 80℃, carrier gas flow rate: 1.8L/min |
|
Note |
/ |
Chromatogram and Data:
- Blank solution detection chart
2. Mannitol single standard solution detection chart
|
Retention time(min) |
K’ |
Area(mV*s) |
Height(mV) |
Symmetry Factor |
Width (min) |
Number of plates(USP) |
Resolution(USP) |
Selectivity |
|
11.355 |
- |
2691.67212 |
216.02324 |
1.11 |
0.1881 |
20184 |
- |
-- |
3. Detection pattern of sample solution 1 (used for method development)
|
Retention time(min) |
K’ |
Area(mV*s) |
Height(mV) |
Symmetry Factor |
Width (min) |
Number of plates(USP) |
Resolution(USP) |
Selectivity |
|
11.162 |
- |
6483.72754 |
440.26373 |
1.03 |
0.2277 |
13311 |
- |
-- |
4. Sample solution 2 (for testing) detection pattern
|
Retention time(min) |
K’ |
Area(mV*s) |
Height(mV) |
Symmetry Factor |
Width (min) |
Number of plates(USP) |
Resolution(USP) |
Selectivity |
|
11.122 |
- |
5244.35352 |
356.00610 |
1.14 |
0.2275 |
13241 |
- |
-- |
Conclusion:
Using Welch Ultisil® HILIC-Amide (4.6×250mm, 5μm) chromatography column under these chromatographic conditions, the peak shape and retention of mannitol are excellent, with no interference from other substances, meeting the customer's detection requirements.