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Determination of the content of compound sulfamethoxazole in children by Ultisil XB C18

Reference Substance

Column

Ultisil XB-C18(4.6×250mm,5μm)

Mobile Phase

Acetonitrile: water: triethylamine = 200:799:1 (glacial acetic acid adjusts pH to 5.9)

Column Temperature

30 ℃

Detection wavelength:

240nm

Flow Rate

1 ml/min

Injection Volume

10μL

Detector

UV

Elution procedure

Time (min)

 Mobile PhaseA(%)

 Mobile Phase B (%)

0

100

0

35

100

0

 

Note

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