Determination of the content of glucose, xylose, xylitol, glycerin, ethanol, formic acid and acetic acid by Xtimate Sugar-H、
Test Report
Chromatography conditions:
Column |
Xtimate® Sugar-H(7.8×300mm,5μm) |
Mobile Phase |
0.025mol/L sulfuric acid |
Detector |
RID |
Injection Volume |
20 μL |
Flow Rate |
0.6 ml /min |
Column Temperature |
60℃ |
Detector Temperature |
35℃ |
Note |
/ |
Chromatogram and Data:
- Detection chart of test solution
Retention time(min) |
K’ |
Area(nRIU*s) |
Height(nRIU) |
Symmetry Factor |
Width (min) |
Number of plates |
Resolution |
Selectivity |
9.021 |
- |
5.58322e6 |
5.16826e5 |
0.88 |
0.1696 |
15672 |
- |
-- |
9.538 |
- |
5.33978e6 |
4.88282e5 |
0.88 |
0.1710 |
17243 |
1.78 |
1.06 |
10.695 |
- |
2.79663e6 |
2.38377e5 |
0.88 |
0.1807 |
19387 |
3.86 |
1.12 |
12.746 |
- |
2.60034e5 |
2.10973e4 |
0.96 |
0.1958 |
23459 |
6.40 |
1.19 |
13.218 |
- |
1.07675e5 |
8605.80469 |
0.86 |
0.2004 |
24106 |
1.40 |
1.04 |
14.190 |
- |
1.32321e6 |
9.24258e4 |
0.83 |
0.2213 |
22781 |
2.71 |
1.07 |
21.272 |
- |
1.13023e6 |
5.41881e4 |
0.80 |
0.3218 |
24194 |
15.32 |
1.50 |
Conclusion:
Under these chromatographic conditions, Welch Xtimate® Sugar-H(7.8×300mm,5μm) can meet the detection requirements.