Determination of unknown sample content using Ultisil HILIC Amphion II
Test Report
Chromatography conditions:
|
Instrument Info |
Shimadzu LC-20AD |
|
Column |
Ultisil®HILIC Amphion II（4.6×250mm，5um） |
|
Mobile Phase |
Acetonitrile/5.5g/L ammonium acetate (use acetic acid to adjust pH to 6.0) =65/35 |
|
Injection Volume |
5 μL |
|
Flow Rate |
0.8 ml /min |
|
Detector conditions |
Excitation wavelength: 274nm; Emission wavelength: 312nm; |
|
Column Temperature |
50℃ |
|
Note |
/ |
Chromatogram and Data:
- Detection amplification chart of mixed standard solution
|
Retention time(min) |
Area |
Height |
Number of theoretical plates(USP) |
Tailing Factor |
Resolution(USP) |
|
8.809 |
8125562 |
684851 |
14662 |
1.234 |
-- |
2. Detection chart of test solution
|
Retention time(min) |
Area |
Height |
Number of theoretical plates(USP) |
Tailing Factor |
Resolution(USP) |
|
8.804 |
9418389 |
803126 |
14552 |
1.210 |
-- |
Conclusion:
Under these chromatographic conditions, Welch Ultisil®HILIC Amphion II（4.6×250mm，5um）can meet the detection requirements.