Cefotiam Hydrochloride-related Substances on Xtimate C18

Content Determination

Column Welch Xtimate C18 4.0x125mm,5μm (P/N: 00101-21031)
Mobile Phase Phosphate buffer (take about 800ml 0.05mol/l disodium hydrogen phosphate solution, adjust pH to 7.7 with 0.05mol/l potassium dihydrogen phosphate solution)-acetonitrile= 88:12
Detector 254nm
Temperature 25℃
Flow Rate 1ml/min
Injection Volume 20ul