Knowledge Base
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Why “Peak Area” is Used for Quantification in Chromatography
Peak area is the preferred metric for quantification in chromatography due to its consistency across variable peak shapes and resistance to signal distortion. Unlike peak height or volume, it offers reliable integration even with minor peak deformation or interference, making it the most robust choice in routine analysis. -
Best Practices for Mobile Phases in HPLC: Preparation, Use, and Storage
Proper preparation, handling, and storage of HPLC mobile phases are essential for achieving accurate, consistent results. This article outlines key considerations for selecting solvents, preparing mobile phases, and avoiding common pitfalls that can affect chromatographic performance and instrument longevity. -
HPLC Content Determination Method Validation: A Comprehensive Guide
This guide provides a detailed overview of HPLC method validation for content determination, highlighting key procedures, technical considerations, and common pitfalls to ensure accurate and reliable results. -
Chromatographic Separation Strategies for Multi-Component Analysis
Mass spectrometry simplifies multi-component analysis, but chromatographic separation remains essential for challenging cases. This article explores enhancements in gradient elution, column selection, temperature, flow rate, and mobile phase composition for separation efficiency and resolution. -
Gradient Optimization in Liquid Chromatography
In this article, we explore key considerations in gradient method development, and witness how optimizing gradient elution in HPLC is key to achieving efficient separation through a real-world case study. -
Mastering the HPLC Autosampler: This Guide is All You Need
The HPLC autosampler is a key component for precise and efficient chromatography. This guide covers autosampler designs, injection methods, and essential maintenance practices to ensure accurate and reliable results. -
A Comprehensive Guide to Guard Columns and Pre-Columns
This guide explores key differences, functions, replacement guidelines, and selection criteria of guard columns and pre-columns (in-line filters) to help ensure optimal chromatographic performance and extended column lifespan. -
A Simple Yet Effective Trick for Isomer Separation
In this article, we explore a simple yet effective trick in the elution program for isomer separation, with two examples to show how this trick works. -
Key Concepts and Considerations of Preparative Liquid Chromatography
In this article, we discuss 10 essential aspects of preparative liquid chromatography, including definition, applications, workflow, key parameters, classification, difference from analytical LC, and practice. -
Unraveling the Case: Reasons Behind Poor LC RSD
A real-world case of poor reproducibility in LC analysis, with a general conclusion of common issues that cause RSD to be high. -
Issues and Solutions to the Use of Ion-Pairing Reagents
Explore the mechanism of ion-pairing reagents, and common issues and solutions when using ion-pairing chromatography. -
Amino (NH2) column: How to keep high performance?
Introduce the principle of amino columns (nh2 columns) and the guidelines for optimizing amino column performance in reversed-phase and normal-phase mode.
