Welchrom PLR 96-well plates, 50mg/2mL, 1pcs/pk

Solid Phase Extraction (SPE) is a widely used and increasingly popular sample preparation technique. It is mostly used to processliquid samples, extracting, concentrating, and purifying semi-volatile and non-volatile compounds, though it can also be appliedto solid samples, which need to be converted into liquids first. At present, SPE is primarily used in the field of food safety, suchas for the analysis of antibiotic and antimicrobial residues in various food products, pesticide residue analysis in agriculturalproducts, and the detection of legal and illegal additives in foods. In the pharmaceutical research field, SPE is widely applied indrug metabolism and pharmacokinetics studies, as well as in the analysis of traditional Chinese medicine. In environmentalprotection, it is used to analyze polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), various pesticides, and organic substances in drinking water, groundwater, and wastewater.

Welchrom SPE Introduction

Welchrom Polymeric SPE

Welchrom polymer based packing materials have overcome the shortcomings of traditional silica matrix packingmaterials, and the use proportion of SPE has been increasing gradually.
Welchrom SPE polymer matrix has the following advantages over silica matrix:

1. Broad pH Range: They are suitable for a wide pH range (0-14) and compatible with most organic solvents.
2. No Active Hydroxyl Groups: The polymer surface lacks active hydroxyl groups, eliminating the secondary adsorption effectsthat can lead to reduced recovery rates of basic compounds.
3. High Adsorption Capacity: The polymer matrix has a high adsorption capacity for most organic substances and higherrecovery rates, with easy quantitative elution and better reproducibility of analytical results.
4. Low Detection Limits: The high recovery rate and capacity reduce detection limits and the amount of polymer adsorbent needed.
5. No Hydrolysis Contamination: The polymer adsorbent does not contaminate expensive extracts due to the hydrolysis of bonded phases.
6. High Interference Stability: Polymer matrix extraction columns can be re-wet if they accidentally dry out during sample preparation, maintaining their performance without the risk of losing analytes or affecting result reproducibility.

Currently available options include HLB, MCX, P-WCX, PWAX, PSAX, and PS/DVB for sample processing.

Different ion groups bonded to the phenyl rings of Welchrom® HLB result in mixed-mode ion exchange reversed-phase adsorbents:

1. MCX: A mixed-mode strong cation exchange reversed-phase adsorbent, bonded with sulfonic acid groups, combining cationexchange and reversed-phase retention. It is suitable for basic compounds with conjugate acids having pKa values between 2-10,mainly amino compounds.
2. PSAX: A mixed-mode strong anion exchange reversed-phase adsorbent, bonded with quaternary ammonium groups,combining anion exchange and reversed-phase retention. It is suitable for carboxylic acid compounds with pKa values between 2-8.
3. P-WCX: A mixed-mode weak cation exchange reversed-phase adsorbent, bonded with carboxyl groups, combining weakcation exchange and reversed-phase retention. It is suitable for strongly basic compounds with conjugate acids having pKavalues greater than 10, such as compounds containing quaternary ammonium groups.
4. PWAX: A mixed-mode weak anion exchange reversed-phase adsorbent, bonded with piperazine groups, combining weakanion exchange and reversed-phase retention. It is suitable for strongly acidic compounds with pKa values less than 1, such ascompounds containing sulfonic acid or phosphoric acid groups.

Welchrom Silica Based SPE

Welchrom® silica-based SPE products use high-quality, high-purity amorphous silica with an average particle size of 45 µm, anaverage pore size of 60 Å, a pore volume of 0.80 cm³/g, and a specific surface area of 480 m²/g. This type of silica provides moderate resistance and flow rate during extraction, making it an ideal choice. Additionally, the bonding silica uses Welchrom's unique surface treatment process, and the bonding phase employs a more stable trivalent bonding method, ensuring consistent extraction and recovery rates for analytes. Silica or bonded silica remains the most commonly used adsorbent in SPE, with a pHrange of 2-7.5. Silica-based adsorbents offer a wide variety of options. 

Welchrom® SPE includes 13 types of silica-based phases: C18E (end-capped), C18 (non-end-capped), C8, Phenyl, CN, NH2, PSA, SCX, SAX, WAX, PRS, Silica, and Diol.

General properties of silica-based adsorbent:

1. The functional groups bonded to the silica surface primarily determine the retention of target compounds. Depending on thetype of bonded functional groups, the retention mechanisms include reversed-phase retention, normal-phase retention, andion-exchange retention.
2. Silica-bonded adsorbents are stable within a pH range of 2-7.5.
3. Silica-bonded adsorbents exhibit rigidity and do not shrink or expand during solvent transformation, achieving equilibriumquickly in new solvents.
4. The silica matrix consists of spherical silica with a particle size of 40-63 µm, with uniform particle size and smooth surface,allowing solvents to pass through even without pressure. The characteristic pore size of silica-bonded adsorbents is approximately 60 Å, suitable for compounds with a molecular weight less than 15,000. Reversed-phase adsorbents are end-capped, while normal-phase and ion-exchange adsorbents are not.

Welchrom Inorganic SPE

Welchrom® Inorganic SPE Adsorbents are commonly used as normal-phase polar adsorbents. Their polarities and surfaceacidities vary, which leads to different applications, but they are generally used for sample purification before analysis, especiallyfor purifying organic extracts from complex samples. For example, Florisil PR adsorbents are frequently used to purify organicsolvent extracts from plant and animal tissues, particularly in pesticide residue analysis. These adsorbents are also used forsample pretreatment in detecting Sudan dyes and malachite green in food products. Welchrom Inorganic adsorbents are activated under strictly controlled conditions, ensuring effective purification with high andconsistent recovery rates and excellent reproducibility.

Welchrom Mixed Mode SPE

Welchrom Mixed Mode SPE combine two types of stationary phases to utilize various interfacial effects for separating andpurifying analytes. They are particularly useful for extracting basic drugs from biological matrices and analyzing pesticideresidues in biological matrices, where numerous interfering substances are present and difficult to wash away. These mixedadsorbents are designed to handle complex sample matrices effectively.

Welchrom Special SPE

Welchrom special SPE column adsorbents include benzopyrene column, Sudan red column, plasticiser column, tea column and Chinese herbal medicine column.

Welchrom Immunoaffinity Column

Welchrom Immunoaffinity Column is used to separate and purify mycotoxins from samples based on specific reactions betweenantigens and antibodies. Antibodies in the immunoaffinity column suspend in the gel by covalent bonding and specifically adsorb the mycotoxin in the sample. If the sample being tested contains the mycotoxin, the toxin will be captured and bound by the antibody when the sample passes through the immunoaffinity column.

All other substances will be washed out of the immunoaffinity column. Methanol is used as an eluent to elute mycosins from theantibodies.

Welchrom Immunoaffinity Column has features as follows

1. Selection of highly specific and high-affinity monoclonal antibodies ensures high-purity samples.
2. Large cartridge capacity with high antibody content increases sample adsorption and improves purification efficiency.
3. Allows purification of large volume samples, providing excellent concentration for low-concentration samples, thus effectivelyenhancing detection methods.
4. Products are validated by extensive scientific experiments, demonstrating good stability and reliability, with sample recoveryrates of 90%-110%.
5. Highly versatile, suitable for various buffer systems without the need for complex or toxic reagents.
6. Simple and quick operation, with each sample taking only 10-15 minutes. Highly adaptable, requiring no specific experimental environment.
7. Purified samples are suitable for ELISA, HPLC, fluorescence photometry, and other methods.
8.  Complies with national standards.

Application

Detection of mycotoxins in grains, peanuts, grain and oil products, alcoholic beverages, animal feed, traditional Chinesemedicine, spices, and dairy products.

Doprah IC Pretreatment Column

During ion chromatography analysis, samples often contain metal ions or organic impurities. These impurities can interfere withthe detection of target ions and also contaminate the ion chromatography column’s packing material, reducing both the separation performance and lifespan of the column. Sample Preparation Columns for Ion Chromatography are developed based onsolid-phase extraction principles. They use high-purity packing materials and leverage reverse adsorption and ion exchange mechanisms to effectively remove impurity ions and organic substances from the samples. This helps prevent contamination ofthe ion chromatography column and maintains its separation efficiency.

Ion chromatography pretreatment column: IC-H, IC-AG, IC-AG /H, IC-BA, IC-NA, IC-RP, IC-C18, IC-AG /Na, IC-BA /Ag/H

Purification mode

1. Impurity adsorption mode -- purification: In this mode, interfering components bind with the stationary phase, while theanalytes pass through the column without being retained.
2. Target adsorption mode -- enrichment + matrix elimination: In this mode, the analytes bind with the stationary phase, whileinterfering components pass through the column without retention. The analytes are then extracted using a suitable eluent.

Advantages

• Ion exchange capacity: 3.5-4.5 mmol/g, which is 1.3 times that of common products on the market.
• The column has a high loading capacity, ensuring more thorough impurity removal and can be reused multiple times depending on the specific sample conditions.
• The column body uses a unique bonding method, capable of withstanding high pressure to prevent cracking and leakage.
• The ion residue concentration in the IC pre-treatment column is extremely low, ensuring accurate detection.

Brand: Welchrom

SPE Type: 96-well plates

Phase: PLR

Particle Size:

Sorbent Weight: 50mg

Barrel Size: 2mL

Pore Size:

Separation Mode:

Surface Area: