SKU: 00289-33052

Xtimate Bio SEC-300. 5μm, 7.8×300 mm

Regular price $1,700.00 USD
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Xtimate Bio SEC Chromatography Column employs ultrapure silica gel as its matrix for size exclusion chromatography. Its stationary phase consists of ultrapure silica gel microspheres with a uniformly nano-thick layer of hydrophilic polymer bonded to their surfaces.

Welch Materials employs a unique surface modification technology, ensuring the complete and uniform bonding of the polymer nanolayer to the silica gel surface. This extensive coverage significantly reduces the nonspecific adsorption of biological samples within the silica gel packing. The Xtimate Bio SEC column also exhibits excellent stability and batch reproducibility. Its primary application areas include biomolecules such as proteins, nucleic acids, peptides, oligonucleotides, bacteria, viruses, and more.

Features

  • Stationary phase consists of hydrophilic polymer with a uniform nano-thick layer bonded to the surface of ultrapure silica gel microspheres.
  • Available in 5μm and 3μm silica gel microspheres, catering to high throughput testing requirements.
  • Offers products with different pore size parameters to meet diverse needs for the separation of biomolecules.
  • Minimal nonspecific adsorption of biological molecules.
  • Demonstrates excellent stability and reproducibility across batches.

    Applications:

    1. Bovine serum albumin
    Overlap spectra:
    Black: Welch Xtimate Bio SEC-120 column
    Red: SEC column from a certain manufacturer
    Conclusion:

    Xtimate Bio SEC-120 chromatography column, when used for detecting bovine serum albumin samples, exhibited significantly higher sensitivity and response values compared to a certain manufacturer’s SEC chromatography column. Moreover, it demonstrated lower nonspecific adsorption of samples.


    2. Monoclonal Antibody Samples


    Conclusion:

    • Under these chromatographic conditions, the main peak of the monoclonal antibody sample exhibits excellent peak shape.
    • The separation between the main peak and the preceding unknown peak is satisfactory, meeting the detection requirements.

     

    3. Human Granulocyte Colony-Stimulating Factor Sample

    Conclusion:

    • Under these chromatographic conditions, the main peak of the human granulocyte colony-stimulating factor sample exhibits excellent peak shape.
    • The separation between the main peak and the surrounding unknown peaks is satisfactory, meeting the detection requirements.

    Brand: Xtimate

    Phase: Bio SEC-300

    Particle Size: 5μm

    Pore Size: 300Å

    USP Classification: L33/L59

    Separation Mode: Size Exclusion

    pH Range: 2.0-8.0

    Surface Area:

    Carbon Load:

    Max.Pressure: 100Bar

    Endcapped:

    Max.Temp.: 5-60°C

    Particle Shape: Spherical

    Product Description

    Xtimate Bio SEC Chromatography Column employs ultrapure silica gel as its matrix for size exclusion chromatography. Its stationary phase consists of ultrapure silica gel microspheres with a uniformly nano-thick layer of hydrophilic polymer bonded to their surfaces.

    Welch Materials employs a unique surface modification technology, ensuring the complete and uniform bonding of the polymer nanolayer to the silica gel surface. This extensive coverage significantly reduces the nonspecific adsorption of biological samples within the silica gel packing. The Xtimate Bio SEC column also exhibits excellent stability and batch reproducibility. Its primary application areas include biomolecules such as proteins, nucleic acids, peptides, oligonucleotides, bacteria, viruses, and more.

    Features

    • Stationary phase consists of hydrophilic polymer with a uniform nano-thick layer bonded to the surface of ultrapure silica gel microspheres.
    • Available in 5μm and 3μm silica gel microspheres, catering to high throughput testing requirements.
    • Offers products with different pore size parameters to meet diverse needs for the separation of biomolecules.
    • Minimal nonspecific adsorption of biological molecules.
    • Demonstrates excellent stability and reproducibility across batches.

      Applications:

      1. Bovine serum albumin
      Overlap spectra:
      Black: Welch Xtimate Bio SEC-120 column
      Red: SEC column from a certain manufacturer
      Conclusion:

      Xtimate Bio SEC-120 chromatography column, when used for detecting bovine serum albumin samples, exhibited significantly higher sensitivity and response values compared to a certain manufacturer’s SEC chromatography column. Moreover, it demonstrated lower nonspecific adsorption of samples.


      2. Monoclonal Antibody Samples


      Conclusion:

      • Under these chromatographic conditions, the main peak of the monoclonal antibody sample exhibits excellent peak shape.
      • The separation between the main peak and the preceding unknown peak is satisfactory, meeting the detection requirements.

       

      3. Human Granulocyte Colony-Stimulating Factor Sample

      Conclusion:

      • Under these chromatographic conditions, the main peak of the human granulocyte colony-stimulating factor sample exhibits excellent peak shape.
      • The separation between the main peak and the surrounding unknown peaks is satisfactory, meeting the detection requirements.

      Specifications

      Brand: Xtimate

      Phase: Bio SEC-300

      Particle Size: 5μm

      Pore Size: 300Å

      USP Classification: L33/L59

      Separation Mode: Size Exclusion

      pH Range: 2.0-8.0

      Surface Area:

      Carbon Load:

      Max.Pressure: 100Bar

      Endcapped:

      Max.Temp.: 5-60°C

      Particle Shape: Spherical

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