Powerful HIC Columns for ADC Characterizartion
Welch Advanchrom HIC-Butyl Column is a high-performance hydrophobic interaction column (HIC), designed for antibody and ADC characterization.
Features:
- Unique chemical design offering excellent selectivity for ADC molecules.
- Ultra-high purity large-pore silica microsphere matrix for superior
column efficiency. - Low non-specific adsorption ensuring high recovery rates.
- Excellent pressure tolerance and durability.
- Outstanding batch-to-batch reproducibility.
- Effective retention and separation using lower salt concentrations.
Product Information:
| Product Name | Advanchrom HIC-Butyl Column | Column Material | Stainless Steel |
| Bonded Phase | Butyl | Dimensions | 4.6×50 mm; 4.6×35 mm |
| Matrix | Ultra-high purity large-pore silica | Max Pressure | 6000 psi |
| Particle Size | 3 μm | Max Temperature | 60°C |
| Pore Size | 1000 Å | pH Range | 2 – 8 |
Applications:
DAR Analysis of Bispecific ADC
Under ammonium sulfate conditions, ADC molecules with different DAR values show effective retention and separation on HIC columns.
Analysis of Monoclonal ADC & Intact Antibody (Rituximab)
Although ammonium sulfate is widely used for DAR determination, it is non-volatile and incompatible with mass spectrometry (MS). A volatile ammonium acetate system was tested, achieving excellent retention and peak shape for both DAR2 ADC and rituximab, providing an MS-compatible solution for future DAR characterization.
Separation of Standard Proteins
Both Welch HIC columns provided excellent retention and separation of the three standard proteins, delivering superior peak shape and theoretical plates compared to competitor columns.
Mobile Phase Recommendation:
Due to the non-volatile nature of conventional ammonium sulfate mobile phase, it is incompatible with mass spectrometry and thus can only be used in HIC-UV systems. For HIC-MS applications, ammonium acetate should be used instead.
Brand: Advanchrom
Phase: Butyl
Particle Size: 3μm
Pore Size: 1000Å
USP Classification:
Separation Mode: HIC
pH Range: 2.0-8.0
Surface Area:
Carbon Load:
Max.Pressure: 6000psi
Endcapped:
Max.Temp.: 60°C
Particle Shape: Spherical
Powerful HIC Columns for ADC Characterizartion
Welch Advanchrom HIC-Butyl Column is a high-performance hydrophobic interaction column (HIC), designed for antibody and ADC characterization.
Features:
- Unique chemical design offering excellent selectivity for ADC molecules.
- Ultra-high purity large-pore silica microsphere matrix for superior
column efficiency. - Low non-specific adsorption ensuring high recovery rates.
- Excellent pressure tolerance and durability.
- Outstanding batch-to-batch reproducibility.
- Effective retention and separation using lower salt concentrations.
Product Information:
| Product Name | Advanchrom HIC-Butyl Column | Column Material | Stainless Steel |
| Bonded Phase | Butyl | Dimensions | 4.6×50 mm; 4.6×35 mm |
| Matrix | Ultra-high purity large-pore silica | Max Pressure | 6000 psi |
| Particle Size | 3 μm | Max Temperature | 60°C |
| Pore Size | 1000 Å | pH Range | 2 – 8 |
Applications:
DAR Analysis of Bispecific ADC
Under ammonium sulfate conditions, ADC molecules with different DAR values show effective retention and separation on HIC columns.
Analysis of Monoclonal ADC & Intact Antibody (Rituximab)
Although ammonium sulfate is widely used for DAR determination, it is non-volatile and incompatible with mass spectrometry (MS). A volatile ammonium acetate system was tested, achieving excellent retention and peak shape for both DAR2 ADC and rituximab, providing an MS-compatible solution for future DAR characterization.
Separation of Standard Proteins
Both Welch HIC columns provided excellent retention and separation of the three standard proteins, delivering superior peak shape and theoretical plates compared to competitor columns.
Mobile Phase Recommendation:
Due to the non-volatile nature of conventional ammonium sulfate mobile phase, it is incompatible with mass spectrometry and thus can only be used in HIC-UV systems. For HIC-MS applications, ammonium acetate should be used instead.
Brand: Advanchrom
Phase: Butyl
Particle Size: 3μm
Pore Size: 1000Å
USP Classification:
Separation Mode: HIC
pH Range: 2.0-8.0
Surface Area:
Carbon Load:
Max.Pressure: 6000psi
Endcapped:
Max.Temp.: 60°C
Particle Shape: Spherical