News
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Maintenance and Care of Liquid Chromatography Systems — Other Aspects
We previously shared ‘6 Steps to Easily Solve Pump Maintenance, No More Urgent Waiting for Engineers Online.’ Now, we continue to share maintenance knowledge about other modules of the liquid... -
Can Normal-Phase Chromatography Be Combined with Mass Spectrometry?
Recently, I have been involved in a project focused on the development of isomers of lycopene, a natural pigment found in food that belongs to the carotenoid family. Over the... -
6 Easy Steps to Pump Maintenance – No More Waiting for Engineers Online
Liquid Chromatography Instruments are commonly used in our labs, with the pump being a crucial component. Learn how to optimize your liquid chromatography usage and perform effective pump maintenance with... -
Optimizing Phosphate Buffer Solution: Preparation Methods and Case Studies
The function of a buffer solution is to maintain the pH of a solution within a certain range, ensuring that the acidity of the test solution conforms to the specified... -
Does Smaller Particle Size in Chromatography Columns Always Lead to Better Separation Efficiency?
In the development process of liquid chromatography methods, the chromatographic column has the greatest impact on separation efficiency. Parameters of the chromatographic column include: bonded phase, column length, diameter, particle... -
Chromatographic Column Selection in HPLC Method Development (Part 1)
In the development of reversed-phase liquid chromatography (RPLC) methods, the selection of chromatographic columns is generally approached through two methods: one based on practical experience and the other rooted in... -
What is the Dead Volume of Column?
If the dead volume is larger, the space leading to sample diffusion will also expand, which makes it easier to cause widened peak and worse peak shape. So how to... -
Pump Maintenance
Pump maintenance is an important part in the liquid chromatography system module. Actually, many problems in LC systems can be traced back to the pump. Normally, the pump of the... -
Preliminary Diagnosis of Malfunction in Liquid Chromatography Module
As is known to us all, the module malfunction of liquid chromatography instrument is very harmful, which not only wastes time, samples, energy and money, but also seriously interferes with... -
How to Select Guard Columns and Pre-columns?
Inline Filters As in-line filters, pre-columns and guard columns have many similarities and differences simultaneously. ONE How to Differentiate Pre-columns From Guard Columns? Being connected before column, both pre-columns and... -
Q&A for Ion-Pairing Reagent
Ion-Pairing Reagent Ion-pairing reagent, a kind of reagent used in lab, can strengthen the retention of ion samples if added in mobile phase of reverse-phase chromatography. Ion-pairing reagents can improve... -
Tips for Column Storage Before the Holiday
LC Column Flush and store the column according to the instructions. It is recommended to detach the column for storage during holidays. ✦ Reversed-phase column For C18, C8, F-C8, C30,...