18 amino acid on Boltimate C18

Instrument and Reagents

Instrument:

1. Balance (precision 0.1mg), 1 pcs
2. Thermostat water bath, 1 pcs
3. Volumetric flask
4. Test tube (1.5×15cm or 1.5×10cm)
5. Microsyringe (5μL or 10μL), 1 pcs
6. Pipette (1000Μl, 200μL), several pipette tips
7. Sample Mixer, 1 pcs
8. HPLC system and AAA column (4.6 × 250mm, 5μm)