2-carboxylic acid-5-bromopyridine and 2-carboxylic acid-5-chloropyridine test report on Ultisil PFP
2-carboxylic acid-5-chloropyridine solution
| Mobile Phase | ||
| Time（min） | Formate buffer（%） | Methanol (%) |
| 0 | 90 | 10 |
| 15 | 75 | 25 |
| 16 | 90 | 10 |
| 25 | 90 | 10 |
| Column | Welch Ultisil PFP 4.6×250mm，5μm (P/N: 00224-31043) |
| Detector | 254nm |
| Temperature | 35℃ |
| Flow Rate | 1.2 ml/min |
| Injection Volume | 20µl |