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23033 Polypeptide(Selection Bioscience LLC) Test Report on Ultisil XB-C4

Full scale

# RT Area Height Area (%) Plates Tailing factor Resolution

Gradient Program

Time(min) A% B%
0 60 40
40 50 50
41 40 60
46 40 60
47 60 40
Column Welch Ultisil® XB-C4 4.6×250mm,5μm (P/N: 00216-31043)
Mobile Phase A: 20mmol/L potassium dihydrogen phosphate (pH2.5) B: Acetonitrile
Detector 210nm
Temperature 40℃
Flow Rate 1.0ml/min
Injection Volume 10ul