6-Aminopenicillanic acid 6-APA test report on Ultisil ALK-C18
Ultisil®AQ-C18（4.6*150mm，5μm），sample：High concentration impurity reference
| Peak No. | Rt (min) | Type | Width (min) | Area (mAU*s) | Height (mAU） | Area (%) |
| 1 | 8.809 | MM R | 0.1816 | 1949.25049 | 178.84999 | 100.0000 |
| Total | 1949.25049 | 178.84999 |
| Column | Ultisil®ALK-C18（4.6*150mm，5μm） |
| Detector | 220 nm |
| Temperature | 30℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 10ul |
| Notes | / |
Gradient Program
| Time (min) | Buffer（PH7.0)% | Acetonitrile% |
| 0 | 96 | 4 |
| 5 | 96 | 4 |
| 13 | 80 | 20 |
| 15 | 80 | 20 |
| 23 | 60 | 40 |
| 26 | 96 | 4 |
| 35 | 96 | 4 |