9-(2-Phosphonylmethoxypropyl)-adenine on Xtimate C18
Part of the Scale
Gradient Program
| Time(min) | A | B |
| 0 | 95 | 5 |
| 30 | 50 | 50 |
| 35 | 50 | 50 |
| 35.01 | 95 | 5 |
| 45 | 95 | 5 |
| Column | Xtimate® C18, 4.6×250 mm, 5 μm (P/N: 00101-21043) |
| Mobile Phase | Mobile phase A: 0.1M ammonium acetate (adjust pH to 3.8 with acetic acid) Mobile Phase B: methanol/acetonitrile =70/30 |
| Detection | 260 nm |
| Temperature | 30℃ |
| Flow Rate | 1.0 mL/min |
| Injection Volume | 20 µL |