Acanthopanax Injection report on Ultisil XB-C18

Reference

Gradient Program

Time(min) A% B%
0 10 90
20 20 80
30 25 75
40 40 60
50 10 90
Column Welch Ultisil® XB-C18 4.6×250mm,5μm (P/N: 00201-31043)
Mobile Phase A: acetonitrile B: 0.1% aqueous phosphoric acid solution
Detector 220nm
Temperature 30℃
Flow Rate 1.0ml/min
Injection Volume 10ul