Acanthopanax Injection report on Ultisil XB-C18
Reference
Gradient Program
Time(min) | A% | B% |
0 | 10 | 90 |
20 | 20 | 80 |
30 | 25 | 75 |
40 | 40 | 60 |
50 | 10 | 90 |
Column | Welch Ultisil® XB-C18 4.6×250mm,5μm (P/N: 00201-31043) |
Mobile Phase | A: acetonitrile B: 0.1% aqueous phosphoric acid solution |
Detector | 220nm |
Temperature | 30℃ |
Flow Rate | 1.0ml/min |
Injection Volume | 10ul |