Acarbose(official standard) on Ultisil XB-NH2

Sample Solution(10mg/ml)

Column Ultisil XB-NH2, 5μm, 4.6×250mm (P/N 00206-31043)
Mobile Phase Phosphate buffer:Acetonitrile =28:72
Detection 210nm
Temperature 35℃
Flow Rate 2ml/min
Injection Volume 10ul