Adefovir Dipivoxil on Ultisil XB-C8
Gradient Program
| Time（min） | A% | B% |
| 0 | 20 | 80 |
| 5 | 20 | 80 |
| 20 | 100 | 0 |
| 32 | 100 | 0 |
| 35 | 20 | 80 |
| 40 | 20 | 80 |
| Column | Welch Ultisil® XB-C8 4.6×250mm，5μm (P/N: 00202-31043) |
| Mobile Phase | A: 0.05 mol/L potassium dihydrogen phosphate buffer-acetonitrile = 50:50; B: 0.05 mol/L potassium dihydrogen phosphate buffer; |
| Detector | 260nm |
| Temperature | 40℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 10ul |