Adenine Isomer on Ultisil LP-C18
Redissolved solution（improve the speed to 1,5ml/min）
| Column | Welch Ultisil® LP-C18 4.6×150mm，5μm (P/N: 00208-31041) |
| Mobile Phase | Dissolve 0.75g copper sulfate, 0.17g L-phenylalanine and 2.3g ammonium dihydrogen phosphate in 1000ml water Adjust pH to 4.0±0.05 with triethylamine |
| Detector | UV 260 nm |
| Temperature | 25°C |
| Flow Rate | 0.5 ml/min |
| Injection Volume | 10µl |
| Notes | Use mobile phase（keep speed 0.2ml/min overnight）to balance column |