Adenine on Ultisil XB-C18
| Column | Ultisil XB-C18, 4.6×250mm, 5μm (P/N 00201-31043) |
| Mobile Phase | 0.63g L-phenylalanine, 0.47g bluestone, 2.21g ammonium dihydrogen phosphate, dissolve in acetonitrile(38ml)-water(962ml) solution |
| Detection | 274nm |
| Temperature | 25°C |
| Flow Rate | 0.6ml/min |
| Injection Volume | 20µl |
| Operation Time | 25min |
| S-isomer tR | ~0.9 |
| Notes | Over-night mobile phase equilibration at 0.2ml/min |