Ampicillin Capsule on Ultisil LP-AQ
Related Substance Sample Solution
| Column | Ultisil® LP-AQ, 5μm, 4.6×250mm PN: 00259-31043 |
| Detection | 254 nm |
| Temperature | 30 ℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20 µl |
Mobile Phase
The standard requires that the gradient run immediately after ampicillin peak elution. (Ampicillin peak was eluted in 10min)
| Time | A% | B% |
| 0 | 55 | 45 |
| 10 | 50 | 50 |
| 40 | 50 | 50 |
| 55 | 48 | 52 |
| 60 | 48 | 52 |
| 70 | 40 | 60 |