Analginum on Ultisil XB-C18
System adaptability test
| Column | Welch Ultisil XB-C18 4.6×50mm，1.8μm (P/N:00201-11037) |
| Mobile Phase | Methanol-sodium dihydrogen phosphate buffer salt (6g sodium dihydrogen phosphate solution + 1ml triethylamine mixed with water to 1000ml, adjust pH to 7.0 with sodium hydroxide solution ) = 28:72 |
| Detector | 254nm |
| Temperature | 30℃ |
| Flow Rate | 1ml/min |
| Injection Volume | 10ul |
| Notes | The concentration of sodium hydroxide solution is: 0.02M |