Anthocyanin on Ultisil PFP(b)
Mixed standard solution
Gradient Program
| Time（min） | %A | %B |
| 0 | 92 | 8 |
| 5 | 90 | 10 |
| 22 | 90 | 10 |
| 30 | 85 | 15 |
| 35 | 80 | 20 |
| 40 | 65 | 35 |
| 45 | 50 | 50 |
| 50 | 35 | 65 |
| 55 | 30 | 70 |
| 55.1 | 92 | 8 |
| 65 | 92 | 8 |
| Column | Welch Ultisil PFP 4.6×250mm，5μm (P/N: 00224-31043) |
| Mobile Phase | Mobile phase A: 5.0% formic acid in water Mobile phase B: methanol |
| Detector | 530nm |
| Temperature | 30℃ |
| Flow Rate | 0.8 ml/min |
| Injection Volume | 10µl |