Ascorbic Acid on Ultisil XB-NH2
Sample Solution
| Column | Ultisil XB-NH2, 5μm, 4.6×250mm (P/N 00204-31043) |
| Mobile Phase | Acetonitrile:Phosphate buffer=75:25(adjust pH to 3.0with phosphoric acid) |
| Detection | 210nm |
| Temperature | 45℃ |
| Flow Rate | 1ml/min |
| Injection Volume | 20ul |