Aspartic Acid, Threonine, Alanine and α-aminoisobutyric acid on Xtimate C18
Mixed Standards
| Column | Xtimate® C18, 4.6×300mm, 5μm (P/N: 00101-21044) |
| Detection | ELSD: drift tube 115 ℃, carrier gas flow rate of 3.2 L/min |
| Temperature | 40℃ |
| Flow Rate | 1.0 mL/min |
| Injection Volume | 20 µL |
Mobile Phase
| Time(min) | 0.2% heptafluorobutyric acid (%) | Methanol(%) |
| 0 | 100 | 0 |
| 10 | 100 | 0 |
| 20 | 75 | 25 |
| 21 | 100 | 0 |
| 30 | 100 | 0 |