Azithromycin on Ultisil XB-C18
Related substances Determination blank solvent
Gradient Program
| Time(min) | Mobile phase A(%) | Mobile phase B(%) |
| 0 | 75 | 25 |
| 35 | 95 | 5 |
| 64 | 95 | 5 |
| 65 | 75 | 25 |
| 71 | 75 | 25 |
| Column | Ultisil XB-C18 4.6×250 mm, 5 μm, |
| Mobile Phase of related substances Determination |
Mobile phase A: Phosphate buffer (0.05mol/L dipotassium hydrogen phosphate solution, adjust pH to 8.2 with 20% phosphoric acid solution): acetonitrile=45:55 Mobile phase B: Methanol |
| Mobile Phase of Content Determination | Phosphate buffer (0.05mol/L dipotassium hydrogen phosphate solution, adjust pH to 8.2 with 20% phosphoric acid solution): acetonitrile=45:55 |
| Detection | 210 nm |
| Temperature | 30 ℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 50 µl |