Biapenem on Ultisil AQ-C18
Sample Solution(full scale)
| Column | Ultisil AQ-C18, 5μm, 4.6×250mm (P/N 00207-31043) |
| Detection | 220nm |
| Temperature | 25℃ |
| Flow Rate | 0.8ml/min |
| Injection Volume | 20ul |
Mobile Phase
| Time | Phosphate Buffer | Methanol |
| 0 | 97 | 3 |
| 8 | 97 | 3 |
| 30 | 80 | 20 |
| 35 | 80 | 20 |
| 36 | 97 | 3 |
| 45 | 97 | 3 |