Cefalexin Related Substance on Ultisil AQ-C18
Related Substance System Suitability Test
| Column | Ultisil® AQ-C18 4.6×250 mm, 5 μm P/N: 00207-31043 |
| Detection | 220 nm |
| Temperature | 30 ℃ |
| Flow Rate | 1 ml/min |
| Injection Volume | 5 µl |
Mobile Phase
A: 0.2mol/L sodium dihydrogen phosphate (Adjust pH to 5.0 with NaOH) B: Methanol
| Time | A% | B% |
| 0 | 98 | 2 |
| 1 | 98 | 2 |
| 20 | 70 | 30 |
| 23 | 98 | 2 |
| 30 | 98 | 2 |