Cefradine Determination on Xtimate G-10

Blue glucan 2000(Mobile phase B)

Column Welch Xtimate G-10 15x300mm
Mobile Phase A: 0.2M disodium hydrogen phosphate: 0.2M sodium dihydrogen phosphate = 95:5 B: Water
Detector 254 nm
Temperature 30℃
Flow Rate 1.5ml/min
Injection Volume 50ul