Chenodeoxycholic Acid on Ultisil XB-C18
Reference standard
| Rt (min) | K’ | Area (mAU*s) | Height (mAU) | Symmetry Factor | Width (min) | Plates | Resolution | Tailing Factor |
| Column | Ultisil XB-C18 4.6×250 mm, 5 μm |
| Mobile Phase | Methanol: water=80:20(adjust pH to 3.7 with 10% perchloric acid) |
| Detection | RI detector |
| Temperature | Column:35°C, RI detector: 35°C |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20 µl |