Chlorpheniramine Maleate on Xtimate C18

Sample

Column Xtimate C18, 5μm, 4.6×250mm PN: 00101-21043
Detection 225 nm
Temperature 35℃
Flow Rate 1.2 ml/min
Injection Volume 10 µl
Notes Sensitive to inject volumn and solvent

Mobile Phase

A: phosphate buffer B: Acetonitrile

Time (min) A (%) B (%)
0 90 10
25 75 25
40 60 40
45 90 10
50 90 10