Cimetidine in Sodium Chloride Injection on Ultisil LP-C18

Impurity C

Column Ultisil® LP-C18, 3μm, 4.6×250mm P/N: 00208-21043
Detection 220 nm
Temperature 30 ℃
Flow Rate 1.0 ml/min
Injection Volume 50 µl

Mobile Phase

Time A% Methanol%
0-60 100 0
60-65 100-90 0-10
65-85 90 10
85-90 90-100 10-0
90-100 100 0