Cimetidine Test Report on Ultisil Polar RP

Test sample of related substance

Column Welch Ultisil Polar RP 4.6×250mm,5μm (P/N: 00215-31043)
Mobile Phase Methanol-water (measure 0.3 ml phosphoric acid and 0.94 g sodium hexanesulfonate, then dissolve with 1000 ml water)= 240:760;
Detector 220nm
Temperature 30℃
Flow Rate 2 ml/min
Injection Volume 20ul