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Clovidipine Butyrate on Ultisil XB-C18

System adaptive solution

Rt (min) K’ Area (mAU*s) Height (mAU) Symmetry Factor Width (min) Plates Resolution Tailing Factor
Column Ultisil XB-C18 4.6×250 mm, 5 μm
Detection 220 nm
Temperature 35°C
Flow Rate 1.2 ml/min
Injection Volume 50 µl

Mobile Phase

A: 0.05mol/L sodium dihydrogen phosphate (Adjust pH to 4.0 with phosphoric acid)

B: Acetonitrile:Methanol=3:2

Time(min) A% B%
0 55 45
12 38 62
70 38 62
72 55 45
80 55 45