Content of Ciprofloxacin Hydrochloride on Ultisil Plus C18
Reference Solution
| No. | RT | Compound | Area | Height | Area% | Resolution（USP） |
| 1 | 10.678 | Ofloxacin | 211684 | 17105 | 2.010 | -- |
| 2 | 12.169 | Ciprofloxacin | 9772287 | 616709 | 92.812 | 3.901 |
| 3 | 13.529 | Impurity I | 545135 | 32983 | 5.177 | 3.154 |
| Total | 10529105 | 666798 | 100.00 |
| Column | Welch Ultisil Plus C18 4.6×250mm，5μm (P/N: 00260-31043) |
| Mobile Phase | 0.025mol/L phosphoric acid solution: acetonitrile = 87:13 (Adjust pH to 3.0 with triethylamine) |
| Detector | 278nm |
| Temperature | 30℃ |
| Flow Rate | 1 ml/min |
| Injection Volume | 20µl |