Deoxyfluorouridine on Ultisil Polar RP

Reference solution

Column Welch Ultisil Polar RP 4.6×250,5μm (P/N: 00215-31043)
Mobile Phase Water-acetonitrile-methanol(75:5:20)
Detector 269nm
Temperature 35℃
Flow Rate 1.0ml/min
Injection Volume 20ul
Notes Pressure is relatively small at 35℃ than that of room temperature