Determination of Bacitracin by Xtimate C18
Sample
1.1 Full Scale
| Column | Xtimate® C18, 4.6×150 mm, 3 μm |
| Mobile Phase | Acetonitrile/phosphate buffer (pH6.0)/water/methanol =43/100/300/557 |
| Detection | 254 nm |
| Temperature | 28℃ |
| Flow Rate | 1.0 mL/min |
| Injection Volume | 100 µL |