Determination of Clotrimazole by Ultisil XB-C18
System Suitability
| Column | Ultisil XB-C18, 5μm, 4.6×150mm |
| Mobile Phase | Methanol: Buffer(adjust pH to 5.7 with 10% phosphate) =70:30 |
| Detection | 215nm |
| Temperature | 30℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 10ul |
| Note | Adjust mobile phase pH only after methanol and buffer well mixed |