Determination of Colla Corii Asini by Ultisil XB-C18
| Column | Welch Ultisil XB-C18 4.6×250mm，5μm |
| Mobile Phase | A:Acetonitrile-0.1mol / L sodium acetate solution (adjust pH to 6.5 with acetic acid) = 7:93 B:Acetonitrile: water = 4:1 |
| Detector | 254nm |
| Temperature | 43℃ |
| Flow Rate | 1ml/min |
| Injection Volume | 5ul |