Determination of D-Tryp by Ultisil Cellu-D

Mixed Standard Solution

Column Ultisil®Cellu-D, 5μm, 4.6×250mm
Mobile Phase Normal Hexane: Isopropanol: Acetonitrile =85:10:5
Detection 220 nm
Temperature 30 ℃
Flow Rate 0.5 ml/min
Injection Volume 10 µl