Determination of various organic acids using Ultisil OAA (1)
Test Report
Sample Information | |||||
Sample name |
16 organic acids |
No. |
W20190516-001 |
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Sample weight |
/ |
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/ |
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Sample collection date |
201905/16 |
Test period |
2019/05/16-05/24 |
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Sample description |
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Test requirements | |||||
Test ingredients |
16 organic acids |
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Reference standards | |||||
Reference standards |
/ |
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Instrument information | |||||
Test instrument |
High performance liquid chromatograph |
Instrument model |
Thermo Fisher U3000 |
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Chromatography conditions:
Column 1 |
Welch Ultisil® OAA（4.6×300mm） |
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Mobile Phase |
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Column Temperature |
32 ℃ |
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Detect wavelength |
210nm |
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Injection Volume |
5 μL |
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Flow Rate |
0.5ml/min |
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Note |
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Preparation of mobile phase:
0.01mol/L potassium dihydrogen phosphate solution: weigh 1.36g potassium dihydrogen phosphate, dissolve in 1000ml water, adjust pH to 2.5 with phosphoric acid,
filter, and obtain.
Methanol: take chromatographic pure methanol, and obtain.
Preparation of sample solution:
Solvent: water;
Solution preparation: take each reference substance, prepare a mixed solution with water, mix well, and obtain
No. |
Name
|
Figure Retention time |
Concentration (mg/ml) |
No. |
Name
|
Figure Retention time |
Concentration (ug/ml) |
1 |
Oxalic acid |
7.675 |
0.20 |
9 |
Acetic acid |
15.035 |
2.0 |
2 |
Tartaric acid |
8.707 |
1.50 |
10 |
Maleic acid |
20.760 |
0.15 |
3 |
Tartaric acid |
9.073 |
1.50 |
11 |
Citric acid |
22.148 |
5.0 |
4 |
Quinic acid |
9.340 |
0.80 |
12 |
Fumaric acid |
25.472 |
0.06 |
5 |
Formic acid |
10.927 |
0.24 |
13 |
Citraconic acid |
29.033 |
0.2 |
6 |
Pyruvic acid Malic acid |
11.460 |
3.50 |
14 |
Acrylic acid |
31.653 |
0.2 |
7 |
Ascorbic acid |
13.120 |
0.50 |
15 |
Propionic acid |
35.857 |
8.0 |
8 |
Lactic acid |
14.095 |
0.50 |
16 |
Citraconic acid |
39.895 |
0.15 |
Chromatogram and Data:
- 1. Mixed control solution
Serial number |
Peak name |
Retention time (min) |
Peak Area(mAU*min) |
peak height(mAU) |
Number of plates(EP) |
Asymmetry(EP) |
Resolution(EP) |
1 |
Oxalic acid |
7.675 |
40.851 |
378.465 |
33761 |
1.21 |
5.89 |
2 |
Tartaric acid |
8.707 |
37.535 |
316.810 |
35807 |
0.98 |
1.98 |
3 |
Tartaric acid |
9.073 |
8.475 |
73.218 |
37806 |
0.99 |
1.32 |
4 |
Quinic acid |
9.340 |
9.953 |
70.697 |
29417 |
1.37 |
4.23 |
5 |
Formic acid |
10.927 |
24.224 |
73.867 |
6676 |
0.95 |
1.36 |
6 |
Pyruvic acid Malic acid |
11.460 |
38.520 |
245.564 |
33615 |
1.18 |
6.19 |
7 |
Ascorbic acid |
13.120 |
73.721 |
395.865 |
33226 |
0.98 |
3.29 |
8 |
Lactic acid |
14.0895 |
3.561 |
18.295 |
34052 |
1.02 |
2.67 |
9 |
Acetic acid |
15.035 |
20.785 |
80.654 |
22560 |
1.39 |
11.93 |
10 |
Maleic acid |
20.760 |
243.295 |
691.126 |
21848 |
1.94 |
2.35 |
11 |
Citric acid |
22.148 |
73.297 |
189.673 |
20254 |
1.99 |
5.52 |
12 |
Fumaric acid |
25.472 |
137.295 |
368.908 |
30263 |
0.95 |
5.43 |
13 |
Citraconic acid |
29.033 |
197.471 |
432.214 |
25336 |
2.00 |
3.67 |
14 |
Acrylic acid |
31.653 |
125.177 |
279.656 |
32690 |
1.06 |
5.00 |
15 |
Propionic acid |
35.857 |
56.502 |
90.696 |
21147 |
1.89 |
4.90 |
16 |
Citraconic acid |
39.865 |
139.454 |
327.895 |
57221 |
1.24 |
N.a. |
Sum |
|
|
1230.116 |
4033.603 |
475679.00 |
21.18 |
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Conclusion:
Under this chromatographic condition, Welch Ultisil® OAA (4.6×300mm)can meet the detection requirements