Diclofenac on Ultisil F-C8

Indolinone Reference Solution

Column Ultisil® F-C8, 5μm, 4.6×250mm PN: 00222-31043
Mobile Phase 0.15% monopotassium phosphate aqueous solution adjust pH =3.2 with phosphoric acid: Methanol=44:56
Detection 229 nm
Temperature 30 ℃
Flow Rate 1 ml/min
Injection Volume 20 µl