Diosmin on Ultisil AQ-C18

Sample Detection Chromatogram

Column Ultisil® AQ-C18 4.6×100 mm, 3 μm P/N: 00207-21039
Mobile Phase acetonitrile-glacial acetic acid-methyl alcohol-water=2:6:28:66 (pH≈2.08)
Detection 275 nm
Temperature 40 ℃
Flow Rate 1.5mL/min
Injection Volume 10 µl