DNA on Ultisil XB-C18
Sample B1’s HPLC-UV chromatogram
No. | Rt (min) | Area (mAU*min) | Height (mAU) | Plates (USP) | Tailing factor | Resolution(USP) |
Column | Ultisil XB-C18 4.6×250mm, 5μm,300Å (P/N: 00201-33043) |
Mobile Phase | Acetonitrile: Potassium dihydrogen phosphate buffer(Adjust pH to 6.3 with triethylamine) gradient |
Detection | UV 260 nm |
Temperature | 30°C |
Flow Rate | 1.0 ml/min |
Injection Volume | 15 µl |