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DNA on Ultisil XB-C18

Sample B1’s HPLC-UV chromatogram

No. Rt (min) Area (mAU*min) Height (mAU) Plates (USP) Tailing factor Resolution(USP)
Column Ultisil XB-C18 4.6×250mm, 5μm,300Å (P/N: 00201-33043)
Mobile Phase Acetonitrile: Potassium dihydrogen phosphate buffer(Adjust pH to 6.3 with triethylamine) gradient
Detection UV 260 nm
Temperature 30°C
Flow Rate 1.0 ml/min
Injection Volume 15 µl